European EPG Workshop 

Madrid, 16-19 June 2024 

PROGRAM 

Organizers:

Alberto Fereres a.fereres@csic.es
Aránzazu Moreno amoreno@ica.csic.es
Elisa Garzo elisa.garzo@ica.csic.es
Clara Lago clara.lago@ica.csic.es
Jaime Jiménez jaimejimenez@ica.csic.es
Piotr Trebicki piotr.trebicki@mq.edu.au
Daniele Cornara daniele.cornara@uniba.it


Venue: ICA-CSIC (16-19 June 2024). Madrid, Spain.


Day 0: 16 June (at ICA-CSIC) 

18:30h-20:00h Welcome Reception & Registration.

Day 1: 17 June 

8:45-9:00 Registration (ICA-CSIC) 

9:00-10:00 Keynote talk: Introduction to Electronic Monitoring of Insect Behaviour. History, Background & Electronics 

        Keynote Speaker: Prof. W.F. Tjallingii 

10:00-10:30: EPG standard waveforms for different insects (waveform library): Phloem feeders
       Speaker: Prof. Alberto Fereres 

10:30-11:00: Coffee break & Poster Session 

11:00-11:30 EPG standard waveforms for different insects (waveform library): Xylem feeders.
       Speaker: Dr. Clara Lago 

11:30- 12:00 Not-standard EPG waveforms of aphids and waveform features due to device adjustments.
       Speaker: Prof. W.F. Tjallingii 

12:00-12:30. Participant talks  

     12:00-12:15 Insights from EPG with Whiteflies: Position-Fixation Techniques and Tethering  
   Speaker: Lize Braat

     12:15-12:30 EPG as a tool to understand plant/arthropod interaction for crop protection  
   Speaker: Juan Manuel Alba Cano

12:30-14:00 Lunch & Poster Session

14:00-14:30 Wiring setting up EPG Equipment, calibration and computerizer data acquisition in EPG recording.
       Speaker: Dr. Elisa Garzo 

14:30-16:00 Afternoon Hands-on

16:00-16:30 Coffee break & Poster

16:30- 18:00 Afternoon Hands-on (cont.)

 Day 2: 18 June 

09:00 -9:30 EPG Analysis & data processing for phloem feeders
       Speaker: Dr. Elisa Garzo 

09:30-10:00 EPG analysis, data processing and workbooks for xylem feeders.
       Speaker: Dr. Daniele Cornara 

10:00-10:30 Keynote talk. An interactive Platform for efficient automatic analysis of EPG signal of piercing sucking insects combining changepoint detection and Machine learning
       Keynote Speaker: Prof. Bernard Reynaud 

10:30-11:00: Coffee break & Poster Session 

Guidelines for experimental design, data analysis and interpretation
       Speaker: Dr. Aranzazu Moreno 

11:30-12:00: Behavioural response to climate change
       Speaker: Dr. Piotr Trebicki 

12:00-12:45 Participants talks  

   12:00-12:15 Speed and directionality of electrical signal propagation in plants in response to physical stress  
   Speaker: Claudio C. Ramírez

   12:15-12:30 Identification of plant virus proteins responsible for the manipulation of host phenotype and vector behavior  
   Speaker: Quentin Chesnais

    12:30- 12:45 Identification of plant cues involved in Rhopalosiphum padi host alternation  
   Speaker: Rituparna Ghosh

12:45-14:00 Lunch & Poster Session

14:30-16:00 Afternoon Hands-on 

16:00-16:30 Coffee break & Poster 

16:30- 18:00 Afternoon Hands-on (cont.)

Day 3: 19 June 

9:00-9:45 Applications of EPG technique: Transmission of plant pathogens by insect vectors.
       Speaker: Prof. Alberto Fereres 

9:45-10:30: Keynote talk. Applications of EPG technique: Host plant resistance and understanding mode of action of chemical compounds using EPG.
       Speaker: Dr. Beata Gabrys 

10:30-11:00: Coffee break & Poster Session 

11:00-11:30: EPG technique and its broader applications using additional techniques (Cryofixation, CT scan and confocal, stylectomy, video-tracking recording, artificial diets, honeydew clock, etc) 
Speaker: Dr. Jaime Jimenez

11:30-12:00: Participants talks  

   Cellulose synthase gene expression analysis of Solanum lycopersicum exposed to combined effects of drought and TYLCV virus  
   Speaker: Samra Mirzayeva

   Aphids as bioelectrodes to measure changes in plant membrane potential in parallel with physiological changes as a result of pathogen infection  
   Speaker: Torsten Will

12:00-12:30: Questions and Discussion 

12:30-14:00 Lunch & posters 

14:00-14:30 00 Afternoon ‘Hands-on’. EPG experiments coupled with video-tracking recording, cryofixation, artificial diets, honeydew clock  

16:00-16:30 Coffee break & Poster Session 

16:30- 18:00 Afternoon Hands-on 

20:00h Closing dinner  

 

 NOTE : Participants should bring their own laptop to the workshop and download and install the EPG installation software in advance (at: epg.csic.es/downloads)


Posters

Alteration of aphid feeding behavior in sugar beet genotypes results in decreased beet yellows virus transmission

Quentin Chesnais, Camille Gutierez, Alessandra Maia-Grondard, Michael Stange, Sandra Otte, Martin Drucker, Véronique Brault

Does SBR - a new disease of sugar beet - affects the feeding behavior of Pentastiridius leporinus?

Brita Kais, J. Koehler, B. Czarnobai De Jorge, Anna Markheiser, J. Gross

Measuring principles in EPGs: Circuit properties and Ohm’s law relationships

Freddy Tjallingii

Cold treatment of infected plum fruits reduces the transmission of the PPV by aphids

Claudio C. Ramírez, Angélica González-González, Jessica Devia-Parra, Isabel Ramírez-Abarca, Mónica Madariaga

Exploring diurnal rhythms in aphid feeding behavior through the lens of the Electrical Penetration Graph Technique

Daniel Kunk

How do endosymbionts modulate plant virus transmission by aphid vectors?

Patricia Sanches, Consuelo De Moraes, Mark Mescher


 AFTERNOON Hands-On PROGRAM

 

 Day 1: Beginners Program

EPG recording (data acquisition) 

DEMO EPG set up: 

- Equipment parts 

- Noise and Faraday cage 

- Amplifier test*

Aphid, whitefly, spittlebug & stink bug wiring 

- Making an insect electrode (done) 

 - Proper electrode attachment 

- Aphid and plant mounting*

- Making an insect electrode 

- Stylet+-d acquisition software* 

- Start: name, comments & adjustments 

- starting*: 1) Giga ON 

2) ‘insects ON’ 

- Optimal voltage adjustment* 

Day 2: Stylet+ software practice


File conversion 

 - Automatic and comment lines 

 - Manual conversion (separate conversion) 

Analysis software (stylet+a) 

 - Use of day 1 data files or sample files 

 - Moving, zooming and .ppt signal pictures 

 - Exporting signal samples 

- Making a ‘Book of reference signals’

Retrieving EPG data 

 - Cursor use and data grid 

 - Saving the data grid  

- DATA PROCESSING SOFTWARE:

- Excel Workbooks for calculation of EPG variables (3 programs)

- Automatic EPG waveform recognition (2 Programs)

 Day 3: Demonstration: other techniques combinated with EPG

     - Honeydew clock for collecting droplets of plant sap 

     - Cryofixation of aphid stylets 

     - Stylectomy of aphid stylets 

     - Video-tracking recording of spittlebug and aphid feeding 

     - EPG recording in artificial diets  

Bring in any questions or problems  

 

* Program items that can be extended for advanced users: 

- Special plant voltage adjustments

- Life establishing of R- and emf-components 

- Event marks in unused channels 

- Use of the calibration button and emf mode probes (innovation)